Sodium iodoacetate as an antiglycolytic agent in blood samples.
نویسندگان
چکیده
We evaluated the effect of sodium iodoacetate on glycolysis in a series of randomly selected blood samples from patients. Glucose values for serum and for serum with added sodium fluoride (2.5 g/liter) or sodium iodoacetate (2 g and 0.5 g/liter) were compared at room temperature. Respective declines in glucose values averaged 170, 40, 30, and 30 mg/liter after 24 h. Iodoacetate-preserved (0.5 g/liter) samples showed no visible hemolysis. Results of determinations of urea with urease and of other tests on SMA 12/60 (Technicon) panels were unaffected.
منابع مشابه
D, L Glyceraldehyde- Will it be an effective Antiglycolytic Agent?
Aim: This study aimed to compare the effectiveness of D, L Glyceraldehyde (GLY) with Plain Sodium Fluoride (NaF) as an antiglycolytic agent and its interferences in the estimation of Serum creatinine by Modified Jaffe′s method and Serum Sodium and Potassium by ion selective electrode(ISE) method. Materials and Methods: Eighty random blood samples were collected from the OP patient after obtaini...
متن کاملStability of plasma lactate in vitro in the presence of antiglycolytic agents.
The use of plasma lactate to assess metabolic or circulatory impairment requires definition of critical preanalytical and analytical parameters. Stability has been documented for only 15 min after acquisition when samples were collected with fluoride and transported on ice. We examined time elapsed before analysis, storage temperature, and the antiglycolytic agent used to define preanalytical c...
متن کاملEvidence for the Presence of Separate Mechanisms Regulating Potassium and Sodium Distribution in Ulva Lactuca
1. The methods employed in these and preceding (25-27) studies were shown to allow analysis of true cellular sodium and potassium concentrations. 2. The rate of reaccumulation of potassium by potassium-deficient cells is independent of the presence or absence of sodium in the external medium. 3. Phenylurethane (10(-3)M), a photosynthetic and metabolic inhibitor, causes a marked progressive loss...
متن کاملExternal quality assessment in primary health care by using fresh whole blood.
Desktop analyzers and single-test meters used in primary healthcare are mainly calibrated to measure whole blood. To minimize matrix effects of control materials in external quality assessment schemes, we stabilized fresh human EDTA-treated blood with sodium iodoacetate (1.8 mmol/L). The whole-blood based control material was useful for control of hemoglobin and glucose, the two most common ana...
متن کاملAction of metabolic poisons on the isolated retina; relation of histological to biochemical lesions.
NOELL (1952, 1953a, b, 1955) reported that sodium iodoacetate (NaIA), administered intravenously to rabbits, selectively destroyed the visual cells of the retina, an effect he attributed to interference with glycolysis. In previous work (Sorsby, Newhouse, and Lucas, 1957), we investigated the effects on the retina of a wide range of thiol reagents, administered intravenously at maximum tolerate...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Clinical chemistry
دوره 21 12 شماره
صفحات -
تاریخ انتشار 1975